Proteins separation and purification by salt gradient ion-exchange SMB
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چکیده
منابع مشابه
The chromatographic separation and purification of acidic proteins on carboxylated ion exchange resins.
The chromatographic separation of proteins on carboxylated ion exchange resins has been widely employed (1, 2). However, as pointed out previously by several authors (l-6), only proteins with alkaline or neutral isoelectric points have been investigated. This report deals with a systematic study of the behavior of proteins with acid isoelectric points on weak cation exchange resins.l In view of...
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Shirai, Y. & Ohkubo, T. (1954a). J. Biochem., Tokyo, 41, 337. Shirai, Y. & Ohkubo, T. (1954b). J. Biochem., Tokyo, 41, 341. Storey, I. D. E. (1950). Biochem. J. 47, 212. Storey, I. D. E. & Dutton, G. J. (1955). Biochem. J. 59,279. Storey, I. D. E. & Dutton, G. J. (1956). Proc. 3rd Int. Congr. Biochem., Brussel8, 1955, p. 162. Strominger, J. L., Kalckar, H. M., Axelrod, J. & Maxwell, E. S. (1954...
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Enriched stable isotopes of many elements have been widely used in many aspects and such isotopes have been primarily used as the tracer for agricultural and biochemical studies and the availability of the isotopically labeled compounds have been now extensively increasing. Chemical exchange separation of isotopes is based on the equilibrium fractionation of isotopes between two phases, ion exc...
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متن کاملOptimization of azeotropic protein separations in gradient and isocratic ion-exchange simulated moving bed chromatography.
The separation of dilute binary mixtures of proteins by salt aided ion-exchange simulated moving bed (SMB) chromatography is optimized with respect to throughput, desorbent consumption and salt consumption. The optimal flow-rate ratios are analytically determined via an adopted "triangle theory". Azeotropic phenomena are included in this procedure. The salt concentrations in the feed and recycl...
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ژورنال
عنوان ژورنال: AIChE Journal
سال: 2007
ISSN: 0001-1541,1547-5905
DOI: 10.1002/aic.11259